Background: The GLUT-4 isglucose transporter protein in cell membrane. In diabetes mellitus, GLUT-4 is decreasedin cell membrane because of the absence or insufficient sensitivity to insulin. GLUT4 is under control of insulin to transport glucose in skeletal muscle and adipose tissue. depletion of GLUT-4 in skeletal muscle and adipose tissue causes insulin resistance.
Objectives: The objective of this study is to investigate the effects of flavonoid-enriched fraction of bitter herb or Andrographis paniculata (FEFBH) on GLUT-4 translocation in high fructose feed fat rat.
Materials and Methods: The aim of this study was to examined the activity of FEFBH on improving GLUT-4 translocation in rat soleus muscle. The diabetic rats mellitus (DM) model of insulin resistance is obtained by fructose induction, egg yolks and lard for 90 days. The treatment groups were given doses of FEFBH 225 mg/KgBB, 450 mg/KgBB and 900 mg/KgBB. Control group was given metformin. Differences between the groups were determined by one-way ANOVA.
Results: The results showed that the administration of FEFBH could increase GLUT-4 translocation (P <0.05) in insulin resistance DM rats compared with negative controls. Increased GLUT-4 translocation significantly at doses of 900 mg/KgBW
Conclusions: Treatment of FEFBH can increase the amount of GLUT-4translocation in soleus muscle rats.
Andrographis paniculata, GLUT-4, insulin resistance, diabetes mellitus, flavonoid.
