The bacterium Rhizobium is s symbiotic nitrogen fixer and is commonly used as Nitrogenous biofertilizer over the legume fields. The agrochemical application has considerable negative effects over the performance of Biofertilizer microbes. In the present research, four Rhizobium species were isolated from groundnut field which is frequently exposed to the herbicide glyphosate using YEMA medium and their plant growth promoting activities viz., production of Ammonia, IAA, HCN and the solubilization efficiency to solubilize Phosphorus and Zinc were tested. All the four isolates could produce significant amount of Ammonia, HCN and IAA and the herbicide caused moderate effects on the efficiency of production of these compounds. Whereas, no Rhizobium isolate were able to solubilize Zn and Phosphorus with and without the presence of herbicide Glyphosate.