Larvicidal and repellent activities of salvia sclarea medicinal plant essential oil against aedes aegypti (linn.), anopleles stephensi (liston) and culex quinquefasciatus (say.) (diptera:culicidae)

Author: 
Deepa J.,Gokulakrishnan J., Balu Selvakumar and Elanchezhiyan K

To investigate the Larvicidal and repellent activities of Salvia sclarea essential oil against Ae. aegypti, An. stephensi and Cx. quinquefasciatus. Twenty five early third instar larvae of selected mosquitoes were exposed to various concentrations (30- 150ppm) and were assayed in the laboratory by using the protocol of WHO 2005; the 24h LC50 values of the essential oil was determined by probit analysis. Repellent activity was carried out in a net cage (45×30×45 cm2) containing 100 blood starved female selected mosquitoes and were assayed in the laboratory condition by using the protocol of WHO 1996; The essential oil of Salvia sclarea was applied at 2.0, 4.0 and 6.0 mg/cm2 separately in the exposed area of the fore arm. The LC50 and LC90 values of Salvia sclarea essential oil on Ae. aegypti, An. stephensi and Cx. quinquefasciatus larvae in 24 h were 66.13, 71.47, 76.06 and 130.19, 142.63 and 144.28 ppm, respectively. The data is statistically significant at P < 0.05. The repellent activity of Salvia sclarea essential oil was found to be most effective for repellent activity against Ae. aegypti followed by An. stephensi and Cx. quinquefasciatus and a higher concentration of 6mg/cm2 provide100% protection up to 280, 240 and 160 min against Ae. aegypti, An. stephensi and Cx. quinquefasciatus, respectively. From the results it can be concluded the essential oil of Salvia sclarea as an excellent potential agent for controlling selected mosquitoes species.

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