An improved, efficient and modified form of non-enzymatic salting out method of genomic dna isolation from human blood

Author: 
Niraj Kumar Srivastava, Rohan Sharma and Deepak Sharma

The present study was performed to establish an improved, efficient and modified form of non-enzymatic salting out method of genomic DNA isolation from human blood. The human blood samples were collected. DNA extraction from blood samples was performed from the original non-enzymatic salting out method as well as a modified form of this method. The reproducibility and sensitivity of modified form of non-enzymatic salting out method were also analyzed by suitable experimental design. The modified form of non-enzymatic salting out DNA extraction method from blood has provided the 2.8 fold more yield of DNA as compared to the original non-enzymatic salting out method. The purity of DNA (A260/A280 > 1.8) was approximately same in modified form of non-enzymatic salting out method of genomic DNA isolation as compared to the original method of non-enzymatic salting out method of genomic DNA isolation. The quantity of extracted DNA has showed the reproducibility. The least volume of blood was two microlitres, required for extraction of DNA from modified form of non-enzymatic salting out method. By using the modified form of non-enzymatic salting out method, a good quality and quantity of DNA from human blood can be extracted, which is enough to perform the polymerase chain reaction-based analysis of gene polymorphism, diagnostic screening and forensic investigation. The improved method does not add any reagents or any instruments and a slight step modification enhanced the yield of DNA.

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