EFFICIENT PROTOCOL FOR IN VITRO PLANTLET REGENERATION FROM LEAF EXPLANTS OF GREEN GRAM (VIGNA RADIATA) L. WILCZEK

Author: 
Viji Maluventhen* and Maruthupandian Arumugam
Country: 
India
Abstract: 

Efficient protocols have been developed to induce highly proliferative callus subsequent to plantlet regeneration from green gram explants. The age of the
seedlings and type of explants were found to influence the callusing response. 11 day old leaf explants were cultured on MS medium containing 2,4-D and Kn induced highly proliferative callus on 28th day. Subsequently plantlet regenerated was achieved on MS basal medium without plant growth hormones. Regenerated shoots showed 65 % rooting on full strength MS basal medium without any plant growth regulators

KeyWords: 

Green gram, Leaf explants, Callus, MS medium

Volume & Issue: 
Vol. 1, Issue 10
Pages: 
250-255
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